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ccdb selective marker  (New England Biolabs)


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    Structured Review

    New England Biolabs ccdb selective marker
    Ccdb Selective Marker, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 2186 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ccdb+selective+marker/BsaI/pmc12276895-213-10-38
    Average 97 stars, based on 2186 article reviews
    ccdb selective marker - by Bioz Stars, 2026-09
    97/100 stars

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    Marker:

    Article Title: Designed miniproteins potently inhibit and protect against MERS-CoV
    Article Snippet: Plasmid sequences were confirmed through sanger sequences of the insert using T7 and T7-term primers from Azenta. .. Trimerization domains were ordered as eblock fragments (IDT) with a ccdb selective marker (flanked by BsaI cut sites) either at the 5’ or 3’ of the trimerization domain and then inserted into a pET29b+ vector using PaqCI (NEB). .. Briefly, 2:1 molar ratio of trimerization domain:plasmid were ligated at 37°C for 20 minutes in a 10 μl mixture reaction containing: 1 μL T4 ligase buffer (NEB), 0.5 μL PaqCI (NEB), 0.5 μL T4 DNA ligase (NEB) and 0.25 μL PaqCI activator (NEB).

    Article Title: Designed miniproteins potently inhibit and protect against MERS-CoV
    Article Snippet: Plasmid sequences were confirmed through sanger sequences of the insert using T7 and T7-term primers from Azenta. .. Trimerization domains were ordered as eblock fragments (IDT) with a ccdb selective marker (flanked by BsaI cut sites) either at the 5’ or 3’ of the trimerization domain and then inserted into a modified pET29b+ vector using PaqCI (NEB). .. Briefly, 2:1 molar ratio of trimerization domain:plasmid was ligated at 37°C for 20 min in a 10 μl mixture reaction containing: 1 μl T4 ligase buffer (NEB), 0.5 μl PaqCI (NEB), 0.5 μl T4 DNA ligase (NEB) and 0.25 μl PaqCI activator (NEB).

    Article Title: Designed miniproteins potently inhibit and protect against MERS-CoV.
    Article Snippet: Plasmid sequences were confirmed through sanger sequences of the insert using T7 and T7-term primers from Azenta. .. Trimerization domains were ordered as eblock fragments (IDT) with a ccdb selective marker (flanked by BsaI cut sites) either at the 5’ or 3’ of the trimerization domain and then inserted into a modified pET29b+ vector using PaqCI (NEB). .. Briefly, 2:1 molar ratio of trimerization domain:plasmid was ligated at 37◦C for 20 min in a 10 μl mixture reaction containing: 1 μl T4 ligase buffer (NEB), 0.5 μl PaqCI (NEB), 0.5 μl T4 DNA ligase (NEB) and 0.25 μl PaqCI activator (NEB).

    Modification:

    Article Title: Designed miniproteins potently inhibit and protect against MERS-CoV
    Article Snippet: Plasmid sequences were confirmed through sanger sequences of the insert using T7 and T7-term primers from Azenta. .. Trimerization domains were ordered as eblock fragments (IDT) with a ccdb selective marker (flanked by BsaI cut sites) either at the 5’ or 3’ of the trimerization domain and then inserted into a modified pET29b+ vector using PaqCI (NEB). .. Briefly, 2:1 molar ratio of trimerization domain:plasmid was ligated at 37°C for 20 min in a 10 μl mixture reaction containing: 1 μl T4 ligase buffer (NEB), 0.5 μl PaqCI (NEB), 0.5 μl T4 DNA ligase (NEB) and 0.25 μl PaqCI activator (NEB).

    Article Title: Designed miniproteins potently inhibit and protect against MERS-CoV.
    Article Snippet: Plasmid sequences were confirmed through sanger sequences of the insert using T7 and T7-term primers from Azenta. .. Trimerization domains were ordered as eblock fragments (IDT) with a ccdb selective marker (flanked by BsaI cut sites) either at the 5’ or 3’ of the trimerization domain and then inserted into a modified pET29b+ vector using PaqCI (NEB). .. Briefly, 2:1 molar ratio of trimerization domain:plasmid was ligated at 37◦C for 20 min in a 10 μl mixture reaction containing: 1 μl T4 ligase buffer (NEB), 0.5 μl PaqCI (NEB), 0.5 μl T4 DNA ligase (NEB) and 0.25 μl PaqCI activator (NEB).



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    ccdb selective marker  (New England Biolabs)
    97
    New England Biolabs ccdb selective marker
    Ccdb Selective Marker, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ccdb+selective+marker/BsaI/pmc12276895-213-10-38
    Average 97 stars, based on 1 article reviews
    ccdb selective marker - by Bioz Stars, 2026-09
    97/100 stars
    		  Buy from Supplier

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